CAS number : 527-07-1
Molecular Weight: 218.14
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Sodium Gluconate USP
d-Gluconic acid, monosodium salt.
Monosodium d-gluconate---CAS Number: 527-07-1.
Sodium Gluconate contains not less than 98.0 percent and not more than 102.0 percent of C6H11NaO7.
A: A solution (1 in 20) responds to the tests for Sodium.
B: It responds to Identification test B under Calcium Gluconate.
Chloride: A 1.0-g portion shows no more chloride than corresponds to 1 mL of 0.020 N hydrochloric acid (0.07%).
Sulfate: A 2.0-g portion dissolved in boiling water shows no more sulfate than corresponds to 1 mL of 0.020 N sulfuric acid (0.05%).
Lead: Dissolve 1.0 g in 25 mL of water: the limit is 0.001%.
Heavy metals: Dissolve 1.0 g in 10 mL of water, add 6 mL of 3 N hydrochloric acid, and dilute with water to 25 mL: the limit is 0.002%.
Reducing substances: Transfer 1.0 g to a 250-mL conical flask, dissolve in 10 mL of water, and add 25 mL of alkaline cupric citrate TS. Cover the flask, boil gently for 5 minutes, accurately timed, and cool rapidly to room temperature. Add 25 mL of 0.6 N acetic acid, 10.0 mL of 0.1 N iodine VS, and 10 mL of 3 N hydrochloric acid, and titrate with 0.1 N sodium thiosulfate VS, adding 3 mL of starch TS as the endpoint is approached. Perform a blank determination, omitting the specimen, and note the difference in volumes required. Each mL of the difference in volume of 0.1 N sodium thiosulfate consumed is equivalent to 2.7 mg of reducing substances (as dextrose): the limit is 0.5%.
Sodium Gluconate FCC Food Grade
C6H11NaO7 Formula---Weight: 218.14
CAS Number 527-07-1
Sodium Gluconate occurs as a white to tan, granular to fine, crystalline powder. It is very soluble in water, and is sparingly soluble in alcohol. It is insoluble in ether.
Function: Nutrient; sequestrant.
A. A 1:20 aqueous solution gives positive tests for Sodium.
B. Dissolve a quantity of sample in water, heating in a water bath at 60Â° if necessary, to obtain a Test Solution containing 10 mg/mL. Similarly, prepare a Standard Solution of USP Potassium Gluconate Reference Standard in water, diluting to 10 mg/mL. Apply separate 5-L portions of the Test Solution and the Standard Solution on a suitable thin-layer chromatographic plate coated with a 0.25-mm layer of chromatographic silica gel, and allow to dry. Develop the chromatogram in a solvent system consisting of a mixture of alcohol, water, ammonium hydroxide, and ethyl acetate (50:30:10:10) until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the chamber, and dry at 110C for 20 min. Allow to cool, and spray with a spray reagent prepared as follows: Dissolve 2.5 g of ammonium molybdate in about 50 mL of 2 N sulfuric acid in a 100-mL volumetric flask, add 1.0 g of ceric sulfate, swirl to dissolve, dilute to volume with 2 N sulfuric acid, and mix. After spraying, heat the plate at 110C for about 10 min. The principal spot obtained from the Test Solution corresponds in color, size, and Rf value to that obtained from the Standard Solution.
Assay: Not less than 98.0% and not more than 102.0% of C6H11NaO7.
Lead: Not more than 2 mg/kg.
Reducing Substances: Not more than 0.5%, calculated as D-glucose.
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